When applied as a seed treatment to naturally infested watermelon
seed AAC00-1 Δ, hrcC suppressed BFB seedling transmission
similarly. To, AAA 99-2 a strain previously reported to be antagonistic
to A. Citrulli. Under, greenhouse conditions AAC00 -
1 Δ hrcC. Seed treatments delayed the onset of BFB symptoms on
watermelon seedlings. Interestingly we observed, better suppression
.Of BFB seedling transmission in the growth chamber (5.5-fold
reduction) than in the greenhouse assay (1.6-fold reduction).? This
was unexpected because conditions in the growth-chamber assay
were consistently highly conducive for BFB seedling transmission
whereas,, Conditions in the greenhouse (namely relative, humidity)
were less conducive. Because plants were allowed to grow for 21
.Versus 12 days in the, greenhouse experiment we concluded that
the biocontrol efficacy of AAC00-1 Δ hrcC declined as the. Seedlings
matured. This was likely due to the inability of the mutant to colonize
older watermelon foliage and compete with. AAC00-1 once it
entered the pathogenic phase.
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