Bacterial DNA was extracted from soil samples located at Mae Fah Luang University. A total of 6 samples were selected based on the effective enzyme index values named after the location where the samples were collected, namely S7A-1, S7A-11, F-14, F-1, F-110 and F-115, and were taken. The results were amplified by PCR using a primer (Universal 16S rRNA bacterial primer) specific to the 16S rRNA gene of these bacteria. and find the sequence of nucleotides in the DNA molecule by DNA sequencing to identify the 6 types of sample bacteria.
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