Lipid peroxidation assay (Ferric th

Lipid peroxidation assay (Ferric thiocyanate method, FTC)
Inhibition of the peroxidation-mediated degradation of a lipid matrix (linoleic acid as the model compound and 2,2’-azobis(2-methylpropionamidine) dihydrochloride (AAPH), as the free radical initiator) was measured using the ferric thiocyanate method (FTC), following the procedure reported by Olszewska, Presler, and Michel (2012), olive oils with IM < 2, for the 2012/2013 crop season were used.

To a methanolic solution of the olive oil (5.6 mg/ml, 37 μl) and 1.3% (w/v) methanolic linoleic acid (175 μl) in H2O (88 μl) and 0.2 M phosphate buffer (pH 7.0, 175 μl) in a screw-cap vial was added AAPH in buffer (55.3 mM , 25 μl). The control solution was prepared by adding pure MeOH (37 μl), instead of the sample. The vial was incubated at 50 ± 0.1 °C for 24 h in the darkness. After that, an aliquot (30 μl) of the reaction mixture was dissolved in a 3:1 (v/v) H2O–MeOH solution (2.91 ml), and a 10% aqueous solution of NH4SCN (30 μl) and 20 mM FeCl2 in 3.5% HCl (30 μl) were added; after 3 min of incubation at rt, the absorbance was measured at 546 nm against the corresponding blank.

The results are expressed as the percentage of lipid peroxidation inhibition:


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Acontrol refers to the solution containing pure MeOH instead of the sample, and Asample refers to the absorbance of oil-containing solutions.

ผลลัพธ์ (อังกฤษ) 3:

Lipid peroxidation assay (Ferric, thiocyanate method FTC)
Inhibition of the peroxidation-mediated degradation of a lipid. Matrix (linoleic acid as the model compound, and 2 2 '- azobis (2-methylpropionamidine) dihydrochloride (AAPH), as the free. Radical initiator) was measured using the ferric thiocyanate method (FTC), following the procedure reported, by Olszewska. Presler and, Michel (2012),Olive oils with IM < 2 for the, 2012 / 2013 crop season were used.

To a methanolic solution of the olive oil (5.6 mg / ml 37, thermal L). And 1.3% (w / V) methanolic linoleic acid (175 thermal L) in H2O (88 thermal L) and 0.2 M phosphate buffer (pH, 7.0 175 thermal L) in a screw - cap. Vial was added AAPH in buffer (55.3, mM 25 thermal L). The control solution was prepared by adding pure MeOH (37 thermal L), instead. Of the sample.The vial was incubated at 50 edge 0.1 ° C for 24 h in the darkness. After, that an aliquot (30 thermal L) of the reaction mixture. Was dissolved in a 3: 1 (V / V) H2O - MeOH solution (2.91 ml), and a 10% aqueous solution of NH4SCN (30 thermal L) and 20 mM FeCl2 in 3.5% HCl. (30 thermal L) were added; after 3 min of incubation at RT the absorbance, was measured at 546 nm against the corresponding blank.

.The results are expressed as the percentage of lipid peroxidation inhibition:




Acontrol Turn MathJax on refers to the. Solution containing pure MeOH instead of the sample and Asample, refers to the absorbance of oil-containing solutions.
.