DPPH assay as a method of analysis of the ability of a substance to resist oxidation (antioxidant), which uses a reagent is 2, 2-diphenyl-1-picrylhydrazyl. Be the fast way Easier analysis, high precision and accuracy.The principle ofDPPH radical is stable in methanol solvent (methanol) in aqueous solution are purple, which absorb light at the wavelength 515-517 nm (nm)How to react when the DPPH antioxidant • has antioxidant effect. The color of the solution turns yellow to purple by comparison with independent Commander Mu antioxidant used as standard is the BHT, if, for example, has the ability to resist oxidation, high. The intensity of the purple solution is decreased.
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