Conclusion The aim of this study was the development of a green liquid, chromatographic method exploring different parameters. Of greening HPLC. Various octadecyl-grafted silica columns were tested in order to investigate selectivity resolution and,, Efficiency parameter in green conditions for statin separation. Reducing particle sizeof column was an advantage when flow. Rate increased to decrease analysis time with acetonitrile-based mobile phase. However with ethanol as organic modifier. In the, mobile phase this particular behaviour was no longer observed. In order to decrease solvent consumption the concentration,, Limit to avoid detector overload was established. Therefore concentration range used for the quantitative determination. Was adjusted as high as it can limit sample dilution and thus solvent consumption.
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