A novel method for analysis of thre

A novel method for analysis of three active components curcumin, demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L. was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column and detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4–H3PO4 (pH 5.0) (50:50, v/v) was used. Good linearity was obtained in the range of 0.208–41.6, 0.197–39.4, and 0.227–114 μM for curcumin, demethoxycurcumin and bisdemethoxycurcumin respectively. The limit of detection reached up to 10−8 M, which was lower than that by UV detection. The relative standard deviations (RSDs) ranged from 1.06% to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day precision, respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3% to 111% were obtained.

ผลลัพธ์ (อังกฤษ) 3:

A novel method for analysis of three active, components curcumin Demethoxycurcumin and bisdemethoxycurcumin in Curcuma. Longa L. Was developed by HPLC coupled with electrochemical detection. Three curcuminoids were well separated on a C18 column. And detected with high sensitivity. A mobile phase containing acetonitrile and 10 mM Na2HPO4 - H3PO4 (pH 5.0) (50: 50 V /, V). Was used.Good linearity was obtained in the range of 0.208 -, -, 41.6 0.197 39.4 and 0.227 - 114 thermal M for curcumin Demethoxycurcumin and,, Bisdemethoxycurcumin respectively. The limit of detection reached up to 10 − 8 M which was, lower than that by UV, detection. The relative standard deviations (RSDs) ranged from 1.06% to 1.88% for intra-day precision and from 4.30% to 5.79% for inter-day. Precision.Respectively. The proposed method has been applied in real herb sample and recoveries ranging from 86.3% to 111% were obtained.