Verify the gene in Anopheles SOCS mini Thomas PCR process monitoring gene PCR process SOCS to increase the number of genes, SOCS, enough to be validated. In the PCR to use dual polymer pyrolysis specific genes, SOCS is CGTCGTACGTCGTATTGCTC-3 ' AcSOCSgsp: 5′ Fwd and Rev AcSOCSgsp: 5′ CGGAAGTACAATCGGTCGTT3 ' command, where reaction by polymer pyrolysis PCR as follows: (1) initial denaturation step at 95° c for 5 min (2) step at 94° c, denaturation for 10 seconds (3) stage degrees 58 annealing at 20 seconds (4) the extension procedure 72 deg c for 30 and a final extension step (5), at 72° c for 10 seconds will automatically repeat a cycle of 40 using a PC of anything (Livak and Schmittgen, 2001).
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