The in vitro antimalarial activitie

The in vitro antimalarial activities against Plasmodium falciparum K1 of four extracts from the stembark
of Picrasma javanica Bl; ie water, methanol, chloroform and hexane extracts were studied using a modification
of the [3
H]hypoxanthine incorporation method. It was found that the hexane extract showed in vitro antimalarial
activity with IC50 of 3.3 μg/ml. The extract was further fractionated using quick column chromatography, resulting
in ten fractions. Fraction V was the most effective against P. falciparum K1 with IC50 of 4.4 μg/ml. Further
isolation of fraction V using a column chromatographic technique provided six fractions. According to 1
H- and 13C-NMR spectra, it could be concluded that the major compound in fraction V-3 was β-sitosterol. Unfortunately,
the antimalarial activity of β-sitosterol could not be determined because of its low solubility in DMSO. However,
fractions V-2 and V-4 still showed in vitro antimalarial activities with IC50 of 2.8 and 3.4 μg/ml, respectively. The
further fractionation of these two active fractions could lead to promising candidates as antimalarial agents.

ผลลัพธ์ (อังกฤษ) 2:

The Plasmodium falciparum in vitro antimalarial activities against K1 of Four extracts from the Stembark
of Picrasma javanica Bl; IE Water, methanol, chloroform and hexane extracts were studied using a modification
of the [3
H] Hypoxanthine Incorporation method. It was that the hexane Extract Found in vitro antimalarial Showed
Activity with IC50 of 3.3 g / ml. The Extract was further fractionated using column chromatography quick, resulting
in Ten fractions. Fraction V was the most effective against P. falciparum K1 with IC50 of 4.4 μg / ml. Further
isolation of fraction V using a column chromatographic fractions Six Technique provided. According to 1
H-NMR and 13C-Spectra, it could be concluded that the Major Compound in beta-sitosterol was fraction V-3. Unfortunately,
beta-sitosterol of the antimalarial Activity could not be determined because of its low solubility in DMSO. However,
fractions V-2 and V-4 still Showed in vitro antimalarial activities with IC50 of 2.8 and 3.4 g / ml, respectively. The
further fractionation of these active fractions could Lead to Two Candidates promising as antimalarial agents.

ผลลัพธ์ (อังกฤษ) 3:

The in vitro antimalarial activities against Plasmodium falciparum K1 of four extracts from the stembark
of Picrasma javanica. Bl; ie, water methanol chloroform and, hexane extracts were studied using a modification
of the [3
H] hypoxanthine incorporation. Method. It was found that the hexane extract showed in vitro antimalarial
activity with IC50 of 3.3 thermal g / ml.The extract was further fractionated using quick, column chromatography resulting
in ten fractions. Fraction V was the. Most effective against P. Falciparum K1 with IC50 of 4.4 thermal g / ml. Further
isolation of fraction V using a column chromatographic. Technique provided six fractions. According to 1
H - and, 13C-NMR spectraIt could be concluded that the major compound in fraction V-3 was β - sitosterol. Unfortunately
the, antimalarial activity. Of β - sitosterol could not be determined because of its low solubility in DMSO. However
fractions, V-2 and V-4 still showed. In vitro antimalarial activities with IC50 of 2.8 and 3.4 thermal g /, ML respectively. The
.Further fractionation of these two active fractions could lead to promising candidates as antimalarial agents.