: Chitin and chitosan have been proved to have enormousapplications in biomedical, pharmaceutical, and industrial fields. The horsemussel, Modiolus modiolus, a refuse of the fishery industries at Thondi, is areserve of rich chitin. The aim of this work is to extract chitosan from thehorse mussel and its further characterization using Fourier transforminfrared spectroscopy (FTIR), micro-Raman spectroscopy, X-ray diffraction(XRD), and elemental analysis. The result of FTIR studies revealeddifferent functional groups of organic compounds such as out-of-planebending (564 cm−1), C−O−C stretching (711 cm−1), and CH2 stretching(1174 cm−1) in chitosan. The degree of acetylation of the extractedchitosan was observed to be 57.43%, which makes it suitable as abiopolymer for biomedical applications. Prominent peaks observed withmicro-Raman studies were at 484 cm−1 (14,264 counts/s), 2138 cm−1(45,061 counts/s), and 2447 cm−1 (45,636 counts/s). XRD studies showedthe crystalline nature of the polymer, and the maximum peak was observed at 20.04°. Elemental analysis showed a considerabledecrease in the percentage of nitrogen and carbon upon the conversion of chitin to chitosan, while chitosan had a higher percentageof hydrogen and sulfur. The antibacterial activities of chitosan from the horse mussel were found to be efficient at a 200 μg/mLconcentration against all the bacterial strains tested with a comparatively higher antibacterial activity against Escherichia coli (9 mm)
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