In previous work senescence of, rose petal cells has been shown to be.Accompanied by a gradual decrease of membrane fluidity as measured, by.A fluorescence polarization technique. Concomitantly an increase, in the.Free sterol-to-phospholipid ratio was found. Both observations were verified.In this study. Further experiments carried, out on whole tissue and isolated.Protoplasts during senescence revealed that there was no quantitative.Change in the level of free sterols. The content of phospholipids decreased.Without any significant change in their composition. Results from experiments.Measuring the incorporation of 132Plorthophosphate indicated a.Reduced capacity for phospholipid synthesis in senescent cells. Both young.And old tissue showed phospholipase A and D activity the former, increasing.With age.It was concluded that the fluidity of rose petal membranes decreases.With age as a result of a decrease in phospholipid content brought about,,By both reduced synthesis and enhanced degradation. Evidence supporting.The view that the phenomena observed are related specifically to changes.In the plasmalemma is discussed.
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