Synthesis of biodiesel was carried

Synthesis of biodiesel was carried out through enzymatic biocatalysis by the transesterification of natural
waste olive oil (alperujo oil) using a recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL).
The most relevant property of this uncommon substrate is the high content of free fatty acids (FFAs),
making it impossible to produce biodiesel through basic catalysis. The synthesis reactions were carried
out in a solvent-free media using a covalent immobilisation on a glutaraldehyde-treated polymethacrylate
amino-epoxide carrier. Methanol was added by stepwise addition to avoid lipase inactivation. FFAs
effects on the kinetics, yields and stability of biocatalysts were studied. It was concluded that the high
free fatty acids content allows an increasing on the initial reaction rate and it gives more stability to
the immobilised enzyme, demonstrating the efficiency of this new substrate for the biodiesel production

ผลลัพธ์ (แอฟริกา) 1:

Deur ensiematiese sintese van biodiesel uit deur die transesterifikasie Biokatalyse Natuurlike gedra
Olyfolie afval (Alperujo Oil) met behulp van 'n rekombinante 1,3-posisionele Selektiewe Rhizopus oryzae lipase (RROL).
Die betrokke eiendom meeste hiervan is die hoë inhoud van ongewone substraat. vrye vetsure (FFAs),
maak dit onmoontlik om Produseer Basiese Deur biodiesel katalise. Die sintese reaksies is uitgevoer
in 'n oplosmiddel vrye gebruik van 'n Media Kovalente immobilisasie op 'n glutaraldehyde behandel Polymethacrylate
amino-epoksied draer. Metanol is bygevoeg deur stapsgewyse Benewens lipase inaktivering vermy. FFAs
uitwerking op die kinetika, opbrengste en stabiliteit van biokatalisators bestudeer. Dit is die gevolgtrekking gekom dat die Hoë
vrye vetsure inhoud op die aanvanklike reaksie Laat 'n toenemende tempo en dit gee meer stabiliteit aan
die Immobilised ensiem, toon die doeltreffendheid van hierdie nuwe substraat vir die biodiesel produksie.