abstractThis special project aims to sort bacteria, as well as to study the efficiency of oil digestion and production of bio surfactants. A total of 18 isolets that can produce lipase enzymes and bio surfactants, and when tested for preliminary oil digestion efficiency with agar well diffusion, the S3L1 isolet bacteria from the sample soil and G2L were tested for the best digestion of oil after 72 hours. Palm oil and soybean oil are passed and not heated. It found that there were 13 isolets that could use all kinds of oils that passed and were not heated as carbon sources. When isolet-derived water from isolets S3L1 and G2L is tested for digestion efficiency in WA foods with heat-resistant and non-heat-resistant test oils. It was found that feed water derived from both isolet bacteria can digest tributyrin in both heat-resistant and non-thermal forms and has the highest digestion efficiency over 72 hours, and all isolet bacteria can form emulsions with all test substances. Isolet S2L6 provides the highest emulsification index at 62.68 mm, second only to S2L10 and S2L2.2 respectively, and the ability to replace oils from feed water derived from bacteria produce lipase enzymes. When tested against palm oil It was found that isolet-derived water S2L10 had the best oil replacement efficiency and then studied the basic morphology characteristics of bacteria that are best effective in digesting oils and creating two bio surfactants, S3L1. The shape of the verse and G2L are gram-negative bacteria. Spherical shapeKeywords: Lipase enzymes Bio surfactants, forest soils fatty residues oil
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