Check SOCS in Anopheles minimus gene by PCR process true inspection process gene SOCS PC arc to increase the number of SOCS gene. Enough to be able to check it. In the process, will use double pike polymer PCR (with gene SOCS is AcSOCSgsp Fwd: 5 "CGTCGTACGTCGTATTGCTC-3" and AcSOCSgsp Rev:? 5 "CGGAAGTACAATCGGTCGTT3" orders pike polymer from the reaction PCR were as follows: (1) step initial denaturation that 95 degrees Celsius. For 5 minutes (2) step denaturation that 94 C for 10 seconds (3) step annealing that 58 degrees Celsius For 20 seconds (4) step extension that 72 C for 30 and (5) step final extension that 72 degrees Celsius For 10 seconds by will repeat the 40 around using the PC. What (Livak, and Schmittgen 2001).
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