The formation of metal complexes wa

The formation of metal complexes was not
observed during h.p.l.c. separation of the porphyrins,
probably because of the relatively short
time of contact between the metals and the porphyrins.
The h.p.l.c. system is adaptable for the preparative
isolation of porphyrins. EDTA (10mg/
100ml of mobile phase) can be added to the mobile
phase without affecting the retention or resolution of
the porphyrins and the isolated porphyrins are free
of metalloporphyrin contamination.
In laboratories where gradient elution facilities are
not available Table 1 shows the mobile phases
suitable for the separation of the individual group of
isomers by isocratic elution or for separation of all
the isomers by stepwise elution.
The analyses of porphyrin isomers in the urine of
HC and CEP subjects and in the faeces of PCT
subjects are examples of practical applications.
The urine of HC subjects contained a large
amount of coproporphyrins, 98% of which was type
III isomer (Fig. 3a). The urine of CEP subjects
contained excessive uroporphyrins and coproporphyrins,
together with a moderate amount of
pentacarboxyporphyrins (Fig. 3b). The porphyrins
were almost entirely of type I isomers. The faecal
porphyrin excretion of PCT subjects is complex,
being characterized by the presence of excess
heptacarboxyporphyrin (type III) and copro- and
isocopro-porphyrins (Fig. 3c), together with smaller
amounts of uroporphyrins (65% type III, 35% type
I), hexacarboxy- (98% type III) and pentacarboxy-porphyrins.
The pentacarboxy fraction consisted
of the type I isomers and three other
detectable isomers, presumably the different type III
isomers present in the urine of PCT patients as
reported by Smith et al., 1980).
The above examples clearly demonstrated the
ability of the h.p.l.c. system to accurately analyse the
ratios of the porphyrin isomers, even in situations
where one isomer is present in large excess of the
other.

ผลลัพธ์ (อังกฤษ) 2:

Not The Formation of Metal complexes was
observed during hplc The Separation of porphyrins,
Probably Because of The relatively short
time of Contact between The Metals and The porphyrins.
The System is Adaptable for The preparative hplc
Isolation of porphyrins. EDTA (10mg /
100ml of mobile Phase) Can be added to The mobile
Phase Without affecting The retention or resolution of
The porphyrins and The isolated porphyrins are free
of Metalloporphyrin contamination.
In Laboratories Where gradient elution Facilities are
Not Available Table 1 Shows The mobile phases.
The suitable for The Separation of Individual Group of
isomers by Isocratic elution or for Separation of all
The isomers by stepwise elution.
The analyzes of porphyrin isomers in The urine of
HC and CEP subjects and in The faeces of PCT
subjects are examples of Practical Applications.
The urine of subjects HC Contained a Large
amount of Coproporphyrins, 98% of which was type
III isomer (Fig. 3a). The urine of subjects CEP
Contained excessive Uroporphyrins and Coproporphyrins,
Together with a moderate amount of
Pentacarboxyporphyrins (Fig. 3b). The porphyrins
were Almost entirely of type I isomers. The faecal
porphyrin excretion of subjects PCT is Complex,
being characterized by The Presence of Excess
Heptacarboxyporphyrin (type III) and Copro- and
Isocopro-porphyrins (Fig. 3C), Together with smaller
amounts of Uroporphyrins (65% type III, 35% type.
I), Hexacarboxy- (98% type III) and Pentacarboxy-porphyrins.
The Pentacarboxy fraction consisted
of The type I isomers and Three Other
detectable isomers, presumably The different type III
isomers Present in The urine of patients PCT As
Reported by Smith et AL. ., the 1980th).
The above examples clearly demonstrated The
Ability of The hplc System to accurately Analyse The
ratios of The porphyrin isomers, Even in situations
Where One isomer is Present in Large Excess of The
Other.