2.5. Experimental procedure
The pilot-scale experiment comprised four stages:
(a) Inoculation (2 weeks): The anaerobic. Reactor was inoculated
with about 44.2 kg of digestate (corresponding to 8.0 kg DS)
sampled from an MSW digester plant (Table. 4). The digester
was then incubated for 2 weeks with no feeding in order to
allow the stabilization of the microflora and. The biodegradation
.Of the residual organic matter of, the digestate which
was estimated from the biogas production. At the end of this
stage,, The digestate was analyzed for dry matter and volatile
matter contents pH and, VFAs.
(b) Start-up stage at a constant organic. Load (4 weeks): Every
week a sub-sample, of 8.0 kg of raw waste (corresponding
to 2.0 kg DS) was defrozen and introduced. Into the digester.
.A mass of digestate corresponding to the same mass of dry
solids was firstly removed from the digester in order to
maintain. A constant mass of dry solids in, the reactor corresponding
to an average residence time of 4 weeks (based on
dry solids).? The digestates were analyzed immediately after
sampling. Biogas production and temperature in the reactor
were monitored. Continuously.Gas composition was measured
before each loading / unloading operation.
(c) Recovery stage (4 weeks): The previous stage. Induced a
strong inhibition of the digestion process. It was therefore
decided to stop feeding the digester for a, while. No waste
was introduced into the digester over 4 weeks in the aim
of allowing the microflora to recover from the state of. Inhibition.
.Biogas production was monitored continuously and
its composition was measured each week. At the end of this
stage the,, Digestate was analyzed to verify that its characteristics
were adequate (pH near 7 - 8 and low concentration of
VFA)
(d). Anaerobic digestion stage with adapted organic load (8 weeks):
After the, recovery stage the digestion was restarted with. A
.Progressive increase of organic load (2.0 kg of raw waste
introduced the first week 4.0 kg, the second week and
6.0 kg. Per week in the last 6 weeks) while digestate was
taken out to maintain constant the mass of dry solids in
the, reactor. The digestates were analyzed immediately after
sampling. Biogas production and temperature in the reactor
were monitored. Continuously.Gas composition was measured
before each loading / unloading operation.
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