The microdilution plates were incubated at 28°C and were examined after seven days of incubation. Minimum inhibitory concentration was defined as the lowest concentration of the oil at which the tested microorganisms did not show visible growth.
Minimum fungicidal concentration (MFC) was determined using the microdilution method(20). Aliquots of 20µL from the wells that did not show growth in the MIC procedure were transferred to new 96-well plates containing 180µL of SDA. The plates were incubated at 28°C for seven days. The lowest concentration of the oil showing no visible growth in the plates was defined as the MFC.