The objective of this study was to evaluate effects of isobutyrate supplementation on rumen fermentation lactation performance,, And plasma characteristics of dairy cows. Twenty multiparous second fi lial generation (F2) cows of a cross between Chinese. Jinnan Yellow and Holstein cows at 148 edge 4.5 days in milk and 22.3 edge 0.81kg milk production were used in a replicated 4 × 4 Latin. Square experiment.The treatments were: control (without isobutyrate), low (LIB), medium (MIB) and high (HIB) isobutyrate supplementation. Of 20 40 and, 60g per cow, per day respectively. Experimental periods were 30 days with 15d of adaptation and 15d of data. Collection. Dry matter (DM) intake was not affected by increasing, isobutyrate supplementationBut milk yields were highest for the 40g / D isobutyrate, supplementation level where proportion of milk fat true protein,, And lactose were minimized. Ruminal pH (6.38 - 6.24) and ammonia N (13.8 - 11.1mg / 100ml) were linearly (P < 0.01), decreased whereas. Total VFA concentration (124 - 131mM) increased at a decreasing rate with increasing isobutyrate supplementation.The ratio of acetate to propionate increased linearly (P < 0.01) from 2.77 to 4.43 as isobutyrate supplementation increased. Due to the increase in acetate production and decrease in propionate production. Digestibilities of OM in
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