Sample preparation and storageSingl

Sample preparation and storage
Single strength orange juice (11.2 Brix, pH 3.7, titratable acidity
0.8%) was prepared by reconstituting frozen Brazilian orange

concentrate (65 Brix) with water (1:5, w/w). The juice was immediately
subjected to pasteurisation (92 C, 30 s) followed by filling
at 85 C into monolayer PET bottles (500 mL), cap twisting and
inversion of the bottles to disinfect the bottle tops. The bottles
were then cooled to ambient temperature by submerging them
into a circulating and chlorinated water tank. After processing,
the juices were stored at 20 and 28 C for 32 weeks, at 35 C for
12 weeks and at 42 C for 8 weeks, in dark incubators (IPP500;
Memmert, Schwabach, Germany).
At each sampling time (Fig. 1), three bottles were taken from
storage, mixed and divided uniformly into smaller tubes
(+30 mL). These tubes were immediately frozen in liquid nitrogen
and stored at 80 C until use. At the time of analysis, tubes were
thawed in a circulating water bath at 25 C.
2.2. C

ผลลัพธ์ (แอฟริกา) 1:

Voorbereiding en berging monster
Orange Juice Enkellopend sterkte (11,2 Brix, pH 3,7, titreerbare suur
0,8%) is opgestel deur die Brasiliaanse hersamestelling Frozen Orange Konsentreer (Brix 65) met water (1: 5, W / W). Die sap is onmiddellik blootgestel aan Pasteurisasie (92 C, 30 s), gevolg deur die invul mono PET bottels op 85 C Into (500 ml), Cap draai en inversie van die bottels na die bottel tops Ontsmet. Die bottels is dan afgekoel deur onderdompeling hulle omgewingstemperatuur en gechlorineerde water in 'n sirkuleer tenk. Verwerking na, is die sap gestoor 20 en 28 C vir 32 weke, op 35 C vir 12 weke en op 42 C vir 8 weke in Dark broeikaste (IPP500; MEMMERT, Schwabach, Duitsland). By elke voorbeeld tyd (Fig. 1), is drie bottels geneem uit Storage, eenvormig gemeng en verdeel in kleiner Tubes (30 ml). Hierdie buise is onmiddellik Bevrore in vloeibare stikstof en gestoor word op 80 C totdat dit gebruik. Teen die tyd van Analise, was Tubes ontdooi in 'n bad sirkuleer op 25 C. Water 2,2. C