Fungus Aspergillus oryzae classified under the microbes that have accumulated high lipids (oleaginous microorganism) has the ability to create and lipid accumulation within the cells at high levels over 40% of the dry biomass. Depending on the conditions of cultivation of A. oryzae are important in food fermentation. It also has an interest in a variety of substances that are important and highly valued species such as enzymes, essential fatty acids needed. This is because the fungus A. oryzae is safe. Can be used in a variety of substrates. Is growing quickly. A gene transfer system (Transformation system) and the expression of genes (gene expression) a powerful tool which facilitates the development of species with molecular techniques to mold fungus A. oryzae with fiber. In the process, fed with liquid (submerge cultivation) fibers are coherent, called the pellet morphology (morphology) in this way affect the nutrients and oxygen to the fungus. The fungus is in the pellet to get nutrients and oxygen throughout which affect yields of interest. It is reported that when the fungus in liquid medium with the addition of beads. (Microparticle) cells to grow and accumulate lipids increased. Due to the growth in such a short fibers are dispersed (dispersed mycelia) cells get oxygen and nutrients it thoroughly. However, the addition of microparticle Into food increases the cost of production. The fungus is genetically modified to do with morphological techniques in molecular biology have been interested. It is reported that a defect in a gene Chitin synthase B (ChsB) as genes involved in the synthesis of chitin. Which is an important component of cell walls. Affect the morphology of fungi. Cause mold to grow in a short fiber. Therefore, in this study, it is interesting to genetically modified fungus A. oryzae has impaired the function of the gene mutants ChsB to be used as a source for the production of fatty acids with the next higher value. To inhibit the function of genes required Plas Mick specific nucleotide sequences of the genes that are looking to stay on the plasmid. Flanked by the genes in the selection Transformer Mansfield. (Trasformant), in which the research was to create plasmid used to inhibit the function of genes in fungi ChsB A. oryzae by using yeast assembly process by homologous recombination in the yeast Saccharomyces cerevisiae helps to connect the pieces. ChsB gene with a specific gene selection. The technique is simple, fast, and does not rely on specific cutting enzymes (restriction enzyme) and ligase enzymes to cut and connect pieces of DNA. Plasma generated by Mick is made to transfer the fungus A. oryzae to Feng ChsB next to the function of the gene.
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