Bacterial DNA was extracted from the selected soil samples of the University of Malaysia, and it was extracted from the visual indicators (s7a-1, s7a-11, f-14, f-1, f-110, f-115) named in the sample collection place. PCR amplification was carried out by using these 16s rRNA gene-specific primers (universal 16s rRNA primers), and the bacteria in DNA molecules were determined by DNA sequence.
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