The recombinant PHB depolymerase
was purified 4.7-fold from the culture broth of E.
coli JM109 pKKPHB. cells, with a yield of 45%.
5.5 mg of PHB depolymerase was obtained from 5
liters of culture, that is, almost the same as the
amount from the culture medium of A. faecalis
AE122