Immediately after sacrifice of expe

Immediately after sacrifice of experimental piglets, samples of the distal ileum (total number of samples=48) from 2cm prior to its opening into the cecum were collected and promptly fixed in neutral buffered formalin for 24h at 4◦C. The specimens were then dehydrated in a graded ethanol series, cleared with xylene and embedded in paraffin. After dewaxing and re-hydration, microtome sections (4