The first step in the Gram staining with crystal violet dye color smear cells, bacteria, all cells on a smear of blue or purple due to cell wall
of Gram-positive, thick wall with crystal violet and yellow once more solution
. Iodine into the crystal violet color will be combined with the Crystal structure has become complex (crystal violetiodine complex) makes a good stick ngakhuen
SI Yi later when clear cells, bacteria, with ethyl alcohol 95% of this step. Bacteria, which are fat delete GSM in
. Very fat cell wall components are melted out with alcohol, make a hole wider cell wall color crystals with
leapt against the walls of cells of bacteria, so now delete non-flammable paint nakae. Best of nakae is positive bacterial components of the wall
. Fat cells are small crystals of colors remains tightly (crystal violet, blue or lilac), which later, when dye with
Safranin ( Bacterial cell walls, red) they did formerly deleted GSM color to red, at this stage, it saw a difference
. Between these two groups of bacteria are clearly.
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