Results3.1. Biological parametersIn

Results
3.1. Biological parameters
In Table 1, length and weight are presented for each species and
sex regardless of depth, site and season. A clear sexual dimorphism
was only seen in A. rostratus whereas in L. lepidion a high proportion
of immature fish was present and no sex-related differences in size
were seen in this species nor in C. mediterraneus. B. mediterraneus is
a hermaphrodite species.
3.2. ChEs characterisation
AChE activity was the dominant cholinesterase in fish muscle,
followed by PrChE while the hydrolysis rate using BTC was practically
immeasurable. Moreover, the lack of enzyme inhibition using
iso-OMPA indicated BuChE was not present in all these deep-sea
fish species. There was a significant correlation (p < 0.01) between
AChE and PrChE in each individual species calculated using
either a subsample: C. mediterraneus and B. mediterraneus
(R ¼ 0.769 and 0.885, n ¼ 10, respectively), A. rostratus (R ¼ 0.953;
n ¼ 38) or the totality of the sample size in L. lepidion (R ¼ 0.762;
n ¼ 129). Hence, only the predominant AChE activity was further
monitored. ChEs characterisation was carried out using three selective
inhibitors, which allow the discrimination between cholinesterases.
Eserine inhibited ChEs at the lowest dose of 0.64 mM
(76e82% inhibition) revealing dominance of true ChEs. The specific
AChE inhibitor BW248c51 caused inhibition ranging from 27%
(A. rostratus) to 68% (L. lepidion) also at the lowest dose of 0.64 mM;
and this inhibition increased in a dose dependent manner (Fig. 2).
The inhibitor iso-OMPA, specific for BuChE, did not affect ChE
activity.

ผลลัพธ์ (แอฟริกา) 1:

Resultate
3,1. Biologiese Parameters
In Tabel 1, lengte en gewig vir elke spesie en word aangebied
seks ongeag diepte en seisoen Site. 'N duidelike seksuele dimorfisme
is eers gesien in L. A. rostratus terwyl in Lepidion 'n groot deel
van die onvolwasse Fish teenwoordig was en geen seks verskille in grootte-Verwante
nie gesien in hierdie spesie in C. Mediterraneus. B. Mediterraneus is
'n Hermafrodiet spesies.
3.2. Ches karakterisering
en was die dominante cholienesterase AChE aktiwiteit in Spier Fish,
gevolg deur die hidrolise koers PrChE terwyl die gebruik van BTC was feitlik
onmeetbare. Verder, die gebrek aan ensieminhibisie behulp
ISO-kompak aangedui Buche was nie teenwoordig in al hierdie diepsee
Fish spesies. Daar was 'n beduidende korrelasie (P <00:01) tussen
AChE en PrChE in elke individuele spesies bereken deur
óf 'n subsample: Mediterraneus C. en B. Mediterraneus
(R ¼ 0,769 en 0,885, n ¼ 10, onderskeidelik), A. rostratus (R. ¼ 0,953;
N ¼ 38) of die totaliteit van die monster in grootte L. Lepidion (R ¼ 0,762;
N ¼ 129). Dus, slegs die oorheersende AChE aktiwiteit verder
gemonitor word. Ches karakterisering is uitgevoer met behulp Drie Selektiewe
inhibeerders, wat die diskriminasie tussen cholienesterase. Laat
Eserine geïnhibeer Ches teen die laagste dosis van 0,64 mm
(76e82% inhibisie) onthulling oorheersing van True Ches. Die spesifieke
AChE inhibeerder veroorsaak inhibisie wissel van 27% BW248c51
(A. rostratus) tot 68% (L. Lepidion) ook op die laagste dosis van 0,64 mm
en hierdie dosis afhanklik inhibisie toegeneem in 'n wyse (Fig 2)..
Die inhibeerder. ISO-kompak, spesifiek vir Buche, het geen invloed op CHO
aktiwiteit.