Polymerase chain reaction (PCR) is

Polymerase chain reaction (PCR) is a useful tool for the
molecular characterization of fungi [15]. Many reports indicate
that fungal species with similar morphology can be further
classified based on PCR results [16], [17], [18], [19]. The intraspecies
diversity of several fungi, including formae speciales and
races, has been further distinguished using the PCR approach
[20], [21]. Recent studies showed that the intergenic spacer (IGS)
region of ribosomal DNA (rDNA) is a source of phylogenetic
markers in Fo [22], [23], [24], [25], [26] and that the region
amplified with the general primers FIGS11/FIGS12 is suitable for
the study of populations, including relationships among the Fo
isolates [22], [27]. The objectives of this study were to identify
polymorphisms in the IGS region of rDNA that differentiate
nonpathogenic from pathogenic Fo isolates and to develop a
method to assess the efficacy of nonpathogenic Fo isolates for use
as potential biocontrol agents to manage Fusarium wilt of
cucumber.

ผลลัพธ์ (แอฟริกา) 1:

Polimerase kettingreaksie (PCR) is 'n nuttige instrument vir die
molekulêre karakterisering van Fungi [15]. Baie verslae dui
dat swam spesies met soortgelyke Morfologie Verdere Kan
geklassifiseer gebaseer op PCR resultate [16], [17], [18], [19]. Die Intraspecies
diversiteit van verskeie Fungi, insluitend Formae Speciales en
Races, is onderskei Verdere PCR met behulp van die benadering
[20], [21]. Onlangse studies het getoon dat die intergeniese spacer (IGS)
streek van ribosomale DNA (rDNA) is 'n bron van Filogenetiese
merkers in Fo [22], [23], [24], [25] [26] en dat die streek
versterk met. Die algemene primers FIGS11 / FIGS12 is geskik vir
die studie van bevolkings, insluitend Fo Die verhoudings tussen
isolate [22], [27]. Die doelwitte van hierdie studie was om te identifiseer
polimorfismes in die IGS-streek van rDNA wat onderskei
patogene van nonpathogenic isoleer Fo en 'n te Ontwikkel
Metode te evalueer die doeltreffendheid van Fo nonpathogenic isolate vir Gebruik
as 'n potensiële biobeheeragente te bestuur Fusarium verwelk van
komkommer.