2.3 Pathogen.P. Expansum was originally isolated from symptomatic pear fruits. The pathogen was cultured in a test tube with potato. Dextrose Agar (PDA; con - sisting of 200 ml boiled potato extract 20 g, glucose and20gagarin1Lofdistilledwater), at28 ° Cfor7d.? Conidia were removed by rubbing the surface of the medium with a bacteriological loop and suspending the loop in sterile. Distilled water. The final spore concentration was adjusted to 1 × 104 spores / ml by use of a hemocytometer.
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