Aerial parts of wild and in vitro plants were dried and ground
by pistil and mortar to a soft powder. For aqueous extract, 5 g
of powder was immersed in 100 ml of distilled water; the solution
was heated in a water bath for 30 min at 95 C. For the
methanol extract, 5 g of powder was immersed in 100 ml of
80% methanol and heated at 40 C for 24 h. Then the samples
were centrifuged and the supernatants were transferred to new
tubes and stored at 20 C.