Injection volume of 80 μL of sample into the donor stream (NaCl), which carrier stream to flow with the MBTH and FeCl3 reagent, respectively, by the reaction
acetaldehyde. Reacts with MBTH the form to become a part of MBTH azine compound after being oxidize with FeCl3 become stable intermediate ready to react with azine compound was a formazan dye the color is blue. Then measured at a wavelength of 600 nm.
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