To do this, add a quantity of genes with PCR technique (Sly) Suilysin and pGEM-sly as a Template, then the PCR product into pGEM ® ligate-T Easy vector and Escherichia coli transmitted into DH5 alpha varieties.
By increased gene dosage Suilysin (Sly) by PCR with pGEM-sly as a Template, then ligate PCR product into pGEM ®-T Easy vector and transformed into Escherichia coli strain DH5 alpha.
The increase Suilysin gene (Sly) technique PCR and pGEM-sly is Template then ligate PCR product into pGEM game systems - T And Easy vector transfer into Escherichia coli species DH5 alpha