Therefore, orchid can not be propagated by branching, and tissue culture technology is used to propagate orchid. The effects of growth regulators and suitable organic substances on the increase of protoplast and seedling number under aseptic conditions were studied. The results showed that after 15 days and 30 days at 25±2°C, they were cultured in the greenhouse for 16 hours in the range of sunlight, and cultured in MS BA medium with 2 concentrations. 5 mg/l combined with NAA 0. 5mg/l can improve the peak number, abundance and root formation of organic matter combined with coconut juice and tomato juice, thus showing the best development effect. The results showed that after two months of culture, the MS preparation containing 5% coconut juice and 2% tomato juice had the best induction effect on the early plateau under the condition of 24±2°C and illumination for 16 hours every day. Meanwhile, coconut juice 7. 5% developed with 2% protease, with the largest number of leaves, and the root system developed best with 2% protease.
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