Diagnosis in animals, the direct fluorescent antibody to be used (DFA) test which looks for the presence of rabies virus in brain tissue antigens. Diagnosis in humans, there are a variety of methods is required for testing. 1. it is recommended to confirm a clinical case of rabies through the use of laboratory-based techniques. For post-mortem diagnosis, the gold-standard diagnostic technique is to detect rabies virus antigen in infected tissues, preferably brain smears or touch impressions collected from a biopsy, by fluorescent antibody test (FAT). Other methods for detection of lyssavirus antigens such as direct rapid immunohistochemistry tests are proven to have sensitivity and specificity comparable to the FAT.2.Ante-mortem diagnosis, diagnosis of rabies during life or (by intra-vitam techniques) is difficult, and depends on the address of the virus through the nervous system.3.Intra vitam skin biopsy, diagnosis is detected that people cry some 25-50% output is positive.
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