Not that AC-1 was eliminated from consideration using The restriction
that probiotic strains Need to Resist Lower Phs during
The stomach Transit (Fioramonti, Theodorou, & Bueno, 2,003; Lin
et AL., the 2006th) .This is an important Selection Criterion for probiotic.
microorganisms.
Physiological concentrations of Human Bile Range from 0.3
to 0.5% (v / v) (Zavaglia, Kociubinsky, Perez, & de Antoni, the 1998th).
The Presence of Bile salts at a Concentration of 1% (W / v) did Not.
interfere in The Development of The BFL-9 Strain and Inhibited
The Other Two strains tested. The Highest in percentage inhibition
was observed for The AC-1 Strain in The First 8 h of Incubation.
The B-7 Strain The Most suffered inhibition at 24 h. In Agreement
with The findings of Vinderola and Reinheimer (the 2003rd), these
results demonstrated that Bile Salt was less inhibitory to
bifidobacteria than lactobacilli. The resistance Against Bile salts
to adhere in The intestine Wall is another important Selection
Criterion for probiotic microorganisms (Fioramonti et AL., in 2003).
Knowing that strains Used in Foods encounter adverse conditions
during Industrial processes (eg, osmotic conditions in
The Food Matrix). The Gastro-intestinal and during Transit (eg,
osmotic conditions Due to Change of Diet) (De Dea Lindner et AL.,
2,007), The inhibition of growth Strain Due to The Presence of
different concentrations of NaCl was tested. The Highest Tolerance
to Salt inhibition, in percentage, was observed for The
Strain BFL-9, followed by B-7. Initial AC-1 demonstrated sensitivity
to osmotic Stress, but After 24 h, The Level of Development
was that in The Same As The Control.
The Ability of The strains to Grow at different temperatures
was Determined. The Optimal growth tested Temperature
was 37 ° C for all strains. However, 15 and 45 ° C Incubation temperatures
demonstrated an inhibitory Effect on The Development
of BFL-9 and B-7 strains, respectively. The AC-1 Strain showed
an Ability to remain viable in temperatures Far from ITS Developmental
Optimum.
Table 5 - Strain characteristics, including resistance to pH, growth in The Presence of Bile, NaCl and temperat.
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