Primer selectionTo select the prime

Primer selection
To select the primers used, sequences of the HO gene
regions were downloaded from the NCBI nucleotide database
and aligned using the multiple sequence alignment
program clustal_x (1Æ8) (Thompson et al. 1997). Based
on these alignments, the S. cerevisiae ⁄ S. pastorianus
(ScHO-F and ScHO-R)- and S. bayanus ⁄ S. pastorianus
(LgHO-F ⁄ LgHO-R)-specific primers were designed
(Table 2). The specificity of these primers was verified by
searching for homologous nucleotide sequences in the
GenBank sequence database using the blast search
program (http://www.ncbi.nlm.nih.gov/).

ผลลัพธ์ (อังกฤษ) 2:

Primer Selection
Used To Select The primers, sequences of The HO Gene
Regions were Downloaded from The NCBI nucleotide Database
and Aligned using multiple Sequence Alignment The
Program Clustal_x (1Æ8) (Thompson et AL. 1,997th). Based
on these alignments, The S. cerevisiae / S. Pastorianus
(SCHO-F and SCHO-R) - and S. bayanus / S. Pastorianus
(LgHO-F / LgHO-R) -specific primers were Designed
(Table 2). The specificity of these primers was verified by
searching for homologous nucleotide sequences in The
GenBank Sequence Database The blast search using
Program (Http://www.ncbi.nlm.nih.gov/).