By the properties of the substance inside the column (Stationary phase) can be captured with the ions of opposite charge with binding reactions between the different ions. (Ionic interaction) does not charge or charge the same as a substance that is an ion exchange that is within a column (Ion-exchanger) moves first. Part a substance being contained within the restraint columns will be flushed out when there is a change to the conditions of pH value or replaced with ion strength greater than (Ionic strength) and intercession that substance comes out hard. Khakhem. have concentrate more, als.
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