The results of this research have found that the classification of the hazardous chemicals used in hospital settings in Ayutthaya has all 4 types: gas, flammable liquids. Silicone compound, along Corrosive substances of all 16 types, manual safety in working with hazardous chemicals. Before the trained employees, the average score of the precious samples and standard deviation equal to 13.29 ± 2.21, and after training, the employees, the average score of the precious samples and standard deviation equal to 17.23 ± 2.03, which found that after training is higher than before, training and satisfaction in the Security Guide to working with hazardous chemicals. Average and standard deviation equal to 3.49 ± 0.39 which is in level.
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