Also the apoptosis, in - dex (AI) significantly increased with the concentration gradient compared with that of the control. Group (Fig. 3). This phenomenon was confirmed by flow cytometry assay. After treatment, with 15 30 and 60, thermal, M lupeol the. Apoptotic rates of PCNA-1 cells were 19.15 edge, edge, 3.56 35.87 3.54 and 60.62 edge 2.76%, respectively which were, all higher than. Those of PCNA-1 cells cultured under normal conditions (5.45 edge 1.44%, P < 0.01; Fig. 4). The 60 thermal M lupeol group was the highest. In all experiments. These data suggested that lupeol induced apoptosis in PCNA-1 cells dose dependently.
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