Due to insufficient Chemical Petroleum additives instead of using ether. As a result make up suck the light measured negative because chlorophyll is soluble in petroleum.
Since the chemical is not enough So instead of using petroleum ether As a result, the absorbance is measured to overspend because chlorophyll is soluble in petroleum.
Because of chemical is not enough. Petroleum is used instead of the ether. The absorbance measured negative due to the chlorophyll insoluble in petroleum.